CRISPR-Cas9 technology is a highly efficient gene-editing tool essential for exploring gene function in functional genomics. The GeCKO v2 library, developed by Feng Zhang's team and available through Addgene (IDs: #1000000048, #1000000049), includes 123,411 gRNAs targeting about 19,050 human genes. This resource enables high-throughput screens to identify critical genes in biological processes or diseases by effectively disrupting target genes. Its precision makes CRISPR ideal for studying complex genetic networks, especially in cancer research.
RNA interference (RNAi) uses small RNA molecules to silence genes and is widely used in biological research. Moffat et al. developed lentiviral shRNA libraries targeting transcription factor regulators, accessible via the GPP Web Portal. These shRNAs specifically reduce mRNA expression, decreasing protein production and inhibiting target gene function. Unlike CRISPR, RNAi provides a reversible method for regulating gene expression, ideal for time-dependent or long-term studies.
This study is part of the Sparkle Platform and operates as a dedicated sub-project within its ecosystem